Abstract: To investigate the infection status of high-risk human papillomavirus ( HPV ) in women. Methods: The study group consisting of 515 healthy women was divided into two experimental groups. One was the low-age group ( <40 years old ), and the other was the high-age group ( ≥40 years old ). The infection status of high-risk HPV was determined by the multiplex polymerase chain reaction fluorescence detection technology. The results of the two groups were compared and statistically analyzed. Results: The total high-risk HPV-positive rate for the entire study group was 8.54% (  44/515 ). The positive rate was 3.45% ( 3/87 ) for the low-age group, and 9.58% ( 41/428 ) for the high age group ( P < 0.05 ).  Conclusion: Women over 40 years old have a high risk for high-risk HPV infection. High-risk HPV detection methods must be enhanced to decrease  the incidence and mortality of cervical cancer in women over 40 years old.